Variation in populations of the coastal dune building grassLeymus arenarius in Iceland revealed by endospermal prolamins |
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Authors: | S Greipsson H El-Mayas H Ahokas |
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Institution: | (1) Department of Biological and Environmental Sciences, Troy University, 36082 Troy, AL, USA;(2) Department of Biology, Georgia State University, 24 Peachtree Center Ave., 30303 Atlanta, GA, USA;(3) Plant Breeding Biotechnology, Agrifood Research Finland, FIN-31600 Jokioinen, Finland |
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Abstract: | Variation in the endospermal protein patterns of seeds amongst and between inland and coastal populations of the dune building
grassLeymus arenarius was examined in Iceland. Seeds were collected from six coastal populations and five inland populations in Iceland. Endospermal
proteins (prolamins) of seeds were extracted with Tris-buffered 2-propanol (50%) and β-mercaptoethanol (0.5% v/v). We used
8% and 12% sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) to separate the prolamin protein bands, which
were stained with Coomassie Brilliant Blue R. Coastal populations were differentiated from inland populations on the basis
of high frequency of high molar weight (HMW) (110–150 kg mol−1) prolamins. Coastal populations had significantly higher proportions of their seed with more than two HMW prolamins, than
those of inland populations. Subtle differences were found among the HMW prolamins of coastal and inland populations indicating
limited intrapopulation variation. The dynamic environment of sand dunes probably influences the genetic composition of these
populations. The results suggest that seeds of the inland and coastal populations should be treated separately in sand dune
reclamation and restoration works |
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Keywords: | Coastal sand dune Colonization Ecosystem restoration Endospermal prolamin Salt tolerance SDS-PAGE |
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