| 不同底物驯化氧化亚铁硫杆菌的差异及对煤炭生物脱硫效率的影响 |
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| 引用本文： | 张德伟,周立祥,杨新萍,王世梅.不同底物驯化氧化亚铁硫杆菌的差异及对煤炭生物脱硫效率的影响[J].环境科学,2011,32(1):272-276. |
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| 作者姓名： | 张德伟 周立祥 杨新萍 王世梅 |
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| 作者单位： | 南京农业大学资源与环境科学学院环境工程系,南京,210095 |
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| 基金项目： | 国家自然科学基金重点项目(40930738,21077054); 江苏省自然科学基金项目(BK2008332) |
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| 摘 要： | 分别以FeS2和FeSO4为底物培养驯化嗜酸性氧化亚铁硫杆菌(Acidithiobacillus ferrooxidans)LX5,结果发现以FeS2为底物驯化A.ferrooxidans LX5,在培养20 d时,培养液中LX5的菌体数量达3.0×107个/mL,对FeS2中亚铁及还原性硫的氧化能力均较强.A.fer...
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| 关 键 词： | 嗜酸性氧化亚铁硫杆菌 FeS2 驯化 胞外多聚物 煤炭脱硫 |
| 收稿时间： | 2010/3/30 0:00:00 |
| 修稿时间： | 2010/6/17 0:00:00 |
Difference in Acclimation of Acidithiobacillus ferrooxidans by Various Substrates and Its Effect on Coal Desulfurization Efficiency |
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| ZHANG De-wei,ZHOU Li-xiang,YANG Xin-ping and WANG Shi-mei.Difference in Acclimation of Acidithiobacillus ferrooxidans by Various Substrates and Its Effect on Coal Desulfurization Efficiency[J].Chinese Journal of Environmental Science,2011,32(1):272-276. |
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| Authors: | ZHANG De-wei ZHOU Li-xiang YANG Xin-ping and WANG Shi-mei |
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| Institution: | ZHANG De-wei,ZHOU Li-xiang,YANG Xin-ping,WANG Shi-mei(Department of Environmental Engineering,College of Resources and Environmental Sciences,Nanjing Agricultural University,Nanjing 210095,China) |
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| Abstract: | In this study, Acidithiobacillus ferrooxidans LX5 was cultured in 9K medium with pyrite and ferrous sulfate as the substrates. Results showed that the number of A. ferrooxidans LX5 acclimated by pyrite for 20 d was 3.0 x 10(7) cell/mL in the liquid, and the oxidation activity to pyrite was stronger. A. ferrooxidans LX5 cultured for 48 h was 1.0 x 10(8) cell/mL in medium with FeSO4. Extracellular polymeric substance (EPS) produced from A. ferrooxidans LX5 acclimated by pyrite was 2 395 microg/(10(10) cells) three times as much as that cultured with FeSO4. A high-sulfur coal desulfurization experiment were carried out with A. ferrooxidans LX5 domesticated respectively in medium with ferrous sulfate and pyrite. The results showed that the coal desulfurization rate was up to 72.4% after bioleaching for 13 d by A. ferrooxidans LX5 acclimated by pyrite, at the same time, the desulfurization rate was only 47.2% with A. ferrooxidans LX5 cultured with ferrous sulfate and reached 65.8% in 20 d. Therefore, the desulfurization efficiency can be improved of coal and shorten the desulfurization time when A. ferrooxidans LX5 acclimated with pyrite as the substrate. |
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| Keywords: | Acidithiobacillus ferrooxidans pyrite acclimation extracellular polymeric substance coal desulfurization |
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