Growth response of Chaetoceros calcitrans (Bacillariophyceae) in batch culture to a range of initial silica concentrations |
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Authors: | I Laing |
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Institution: | (1) Fisheries and Food, Directorate of Fisheries Research, Fisheries Experiment Station, Ministry of Agriculture, Benarth Road, LL32 8UB Conwy, Gwynedd, North Wales, UK |
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Abstract: | Batch cultures of the marine unicellular centric diatom Chaetoceros calcitrans (Paulsen) Takano were maintained by serial subculturing every 4 d into nutrient-enriched natural sea-water medium supplemented with 350, 950 and 1 400 g-at Si l-1. The diatom cultures removed initial silica concentrations of 350 and 950 g-at l-1 from the medium within 2 and 3 d, respectively. About 30 g-at l-1 of the highest initial concentration remained in the medium after 4 d. The mean final cell density with an enrichment of 350 g-at Si l-1 was 3.43±0.26×104 cells l-1 (median cell volume = 77.5±5.0 m3); with 950 g-at Si l-1, 8.55±0.55×104 cells l-1 (50.0±4.5 m3); and with 1 400 g-at Si l-1, 9.72±0.48×104 cells l-1 (37.3±5.0 m3). There was no significant difference in the final total organic weight of cells produced, which was in the range of 170 to 190 mg per 250 ml culture. This consisted of proportionately more lipid and carbohydrate and less protein from the treatment with 350 g-at Si l-1 than from the 1 400 g-at Si l-1 treatment. |
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