首页 | 本学科首页   官方微博 | 高级检索  
     

珠江口典型水产养殖区抗生素抗性基因污染的初步研究
引用本文:梁惜梅,聂湘平,施震. 珠江口典型水产养殖区抗生素抗性基因污染的初步研究[J]. 环境科学, 2013, 34(10): 4073-4080
作者姓名:梁惜梅  聂湘平  施震
作者单位:中国科学院南海海洋研究所热带海洋环境国家重点实验室;中国科学院大学;暨南大学生态学系
基金项目:中国科学院-国家外国专家局创新团队国际合作伙伴计划项目(KZCX2-YW-T001);中国科学院知识创新工程项目(KZCX2-YW-Q07);国家自然科学基金项目(41076069,40776086)
摘    要:使用普通PCR和荧光定量PCR方法对珠江口典型水产养殖区水和沉积物中3种磺胺类、7种四环素类、1种喹诺酮类抗生素抗性基因(antibiotic resistance genes,ARGs)和1种整合子基因进行了定性和定量研究.结果表明,除tetW外,所有其他ARGs在珠江口养殖区中均被检出,其中sul1、sul2和int1的检出率为100%.相同养殖模式下,养殖时间越长ARGs的相对含量越高,表明ARGs具有累积效应;不同养殖模式池塘中ARGs的含量存在差异,表明养殖模式可能影响ARGs的含量与分布.int1相对含量与sul1和ARGs总量之间均存在显著相关性(P<0.05),表明int1在ARGs的水平传播中起着非常重要的作用.此外,珠江口水产养殖区沉积物中抗生素浓度与ARGs总量存在显著相关性(P<0.05),说明水产养殖区中抗生素残留是诱导养殖区ARGs的主要因素.

关 键 词:抗生素抗性基因  普通PCR  荧光定量PCR    沉积物  水产养殖区  珠江口
收稿时间:2013-01-30
修稿时间:2013-03-15

Preliminary Studies on the Occurrence of Antibiotic Resistance Genes in Typical Aquaculture Area of the Pearl River Estuary
LIANG Xi-mei,NIE Xiang-ping and SHI Zhen. Preliminary Studies on the Occurrence of Antibiotic Resistance Genes in Typical Aquaculture Area of the Pearl River Estuary[J]. Chinese Journal of Environmental Science, 2013, 34(10): 4073-4080
Authors:LIANG Xi-mei  NIE Xiang-ping  SHI Zhen
Affiliation:LIANG Xi-mei;NIE Xiang-ping;SHI Zhen;State Key Laboratory of Tropical Oceanography,South China Sea Institute of Oceanology,Chinese Academy of Sciences;University of Chinese Academy of Sciences;Department of Ecology,Jinan University;
Abstract:Traditional and quantitative PCR techniques were used to determine the occurrence and quantities of ARGs, including three types of genes resistant to sulfonamide, seven for tetracycline resistance and one for quinolone resistance, as well as one integron gene in typical aquaculture of the Pearl River Estuary. The results showed that all genes except for tetW were detectable in the aquaculture environment, and sul1, sul2 and int1 were the most frequently detected genes (detectable percentage, 100%). Relative abundances of ARGs increased with the prolongation of rearing time under the same aquaculture pattern, suggesting a cumulative effect. Moreover, the occurrences of ARGs in the ponds were different with different aquaculture patterns, indicating that the aquaculture pattern might play an important role in the abundances and distributions of ARGs. Relative abundances of int1, as a horizontal mobile genetic element, were significantly correlated to the levels of sul1 and the total ARGs (P<0.05). The total concentration of antibiotics exhibited a good positive correlation with the total concentration of ARGs in sediments (P<0.05). All results elucidated that extensive residues of antibiotics in the aquaculture substantially increased the abundances of ARGs probably owning to the induction of horizontal gene transfer of ARGs among bacteria.
Keywords:antibiotic resistance genes  traditional PCR  real-time quantitative PCR  water  sediment  aquaculture  Pearl River Estuary
本文献已被 CNKI 等数据库收录!
点击此处可从《环境科学》浏览原始摘要信息
点击此处可从《环境科学》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号