MBR处理猪场废水过程中抗生素抗性基因的去除

以猪场废水中典型的抗生素抗性基因(antibiotic resistant genes, ARGs)为研究对象,针对MBR工艺处理猪场废水,研究生物处理过程抗性基因去除及其与工艺参数的关系。膜生物反应器(membrane bio-reactor, MBR)小试装置对猪场废水中COD和TN的平均去除率分别达到95.5%和92.4%。在污泥龄(sludge retention time,30 d)较长时,MBR对ARGs的拷贝数去除率在1.5~2 logs,而将SRT缩短至15 d时去除率最高可达3.78 logs。环境因子(DO、pH、Temp、SS、COD、NH+₄-N和TN)对MBR出水中ARGs拷贝数和丰度的影响呈现出相同趋势。因此,通过降低SRT、提高DO、pH、和温度等措施,可以提高ARGs拷贝数和丰度的消减效率,具有实际应用价值和指导意义。

REMOVAL OF ANTIBIOTIC RESISTANCE GENES DURING THE TREATMENT OF SWINE WASTEWATER BY MBR

In this study antibiotic resistant genes (ARGs) in swine wastewater during the biological treatment was investigated through the methods of laboratory experiment. The ARGs removal during the biological treatment as well as the parameter optimization were explored, in order to provide the theoretical and technological support for control and elimination of ARGs in swine waste. A lab-scale membrane bio-reactor (MBR) was established to treat swine wastewater. The mean removal rate of COD and TN were 95.5% and 92.4%. At the longer SRT of 30, MBR showed the highest removal efficiency of ARGs copy number between 1.5 and 2 logs,but the removal efficiency can reach up to 3.78 logs when the SRT is shortened to 15 days. The effects of environmental factors(DO、pH、T、SS、COD、NH+₄-N and TN) on the copy number and abundance of ARGs in MBR effluent showed the same trend. Therefore, by reducing SRT, increasing DO, pH, and temperature, the depletion efficiency of ARGs copy number and abundance can be improved, which has practical application value and guiding significance.