1株金霉素降解菌的筛选及基于响应面法的降解条件优化

从河南驻马店金霉素制药厂活性污泥中分离、筛选出1株金霉素降解菌,经16SrDNA鉴定为芽孢杆菌属(Bacillus sp.),命名为JMS-B01。为进一步提高该菌株的金霉素降解率,对其进行降解条件优化。采用正交试验确定显著影响金霉素降解率的3个因素(温度、金霉素浓度、接种量),通过Box-Behnken试验设计及响应面法分析确定最佳降解条件。结果表明:拟合所得的预测值与实际值相关性良好,最佳降解条件为温度34.0℃、金霉素浓度102.5 mg/L、接种量2%(体积分数),在此条件下金霉素降解率可达到96.50%。其中,温度是影响菌株JMS-B01对金霉素降解率的最重要因素之一。

SCREENING OF A CHLORTETRACYCLINE-DEGRADING STRAIN AND ITS DEGRADATION CONDITIONS OPTIMIZATION USING RESPONSE SURFACE METHODOLOGY

A chlortetracycline-degrading strain was isolated from the activated sludge of a chlortetracycline pharmaceutical factory in Zhumadian in Henan. Based on the analysis of its 16S rDNA gene sequence, the isolated strain named JMS-B01 was identified as a member of the genus Bacillus. To improve the chlortetracycline degradation rate, the degradation conditions were optimized. Firstly, three factors playing important roles in the chlortetracycline degradation rate were selected based on the orthogonal test. Then box-Behnken experimental design and response surface method were used to analyze the optimal degradation conditions. The fitting curve had a good correlation with the measured values. The optimal conditions were as follows:temperature of 34.0℃, chlortetracycline concentration of 102.5 mg/L, inoculation amount of 2%(V/V). Under the optimal conditions mentioned above, the chlortetracycline degradation rate reached 96.50%. Temperature was one of the most important factors affecting the chlortetracycline degradation rate of strain JMS-B01.