蒽醌-2-磺酸钠促进Klebsiella oxytocaGS-4-08脱色产氢机制与产能分析

采用0~0.5 mmol·L~(-1)蒽醌-2-磺酸钠(AQS)研究其对Klebsiella oxytoca GS-4-08厌氧降解甲基橙(MO)的影响,对比细菌产氢、降解碳源及生成产物等情况,得出最佳浓度为0.1 mmol·L~(-1).在该浓度下,Klebsiella oxytoca GS-4-08可在10 h内完全脱色MO,25 h内降解蔗糖达到92%,同时产生0.117 mol·mol~(-1)乙醇和0.116 mol·mol~(-1)乙酸,2.25 mol·mol~(-1)氢气;将实验结果与已有研究进行对比,分析了AQS促进Klebsiella oxytoca GS-4-08厌氧脱色MO与产氢的机制.通过将细菌降解过程中的物质转换为COD进行对比,对降解过程中电子转移进行了分析,得到0.1 mmol·L~(-1)AQS浓度时电子得率最高,其为87.98%;并将细菌降解过程中的能源转换进行对比,得到0.1 mmol·L~(-1)AQS浓度时能源产量最高,其为802 k J·mol~(-1);同时得出Klebsiella oxytoca GS-4-08发酵过程可产生两种以上生物燃料,表明了其在工业生产应用上的前景.

Impact of Anthraquinone-2-sulfonic Acid on the MO Decolorization, Hydrogen Production and Energy Creation During Anaerobic Fermentation of Klebsiella oxytocaGS-4-08 with Sucrose

The effect of 0-0.5 mmol·L^-1 anthraquinone-2-sulfonic acid on anaerobic fermentation of Klebsiella oxytocaGS-4-08 was analyzed. By comparing bio-hydrogen production, carbon source degradation, and degradation products, the optimal concentration of 0.1 mmol·L^-1 was acquired. Under this concentration, Klebsiella oxytocaGS-4-08 could decolorize 100% methyl orange within 10 h, degrade 92% sucrose within 25 h, meanwhile produce 0.117 mol·mol^-1 ethanol, 0.116 mol·mol^-1 acetic acid, 2.25 mol·mol^-1 hydrogen. Besides, the promoting mechanism of AQS on MO decolorization and hydrogen production by Klebsiella oxytocaGS-4-08 was analyzed by comparing the experimental results and the literatures. The electron transfer and energy conversion are analyzed during the bacterial degradation process by converting the substances into COD; the optimal electron yield was 87.98% and the optimal energy generation rate was 802 kJ·mol^-1, and both were achieved under 0.1 mmol·L^-1 of AQS. Using Klebsiella oxytoca GS-4-08, at least two types of biofuels could be produced via fermentation process, which showed prospects in future applications.