Abstract: | Article 14 of the Convention on Biological Diversity (CBD) calls for the inclusion of impacts on biodiversity in Environmental Impact Assessment (EIA), which is a significant instrument for site-specific impact prediction. In view of the shortcomings reported for Environmental Impact Statements (EISs), guidelines with indicators could improve the consideration of biological diversity in EISs. This study aims to establish guidelines for the analysis of the inclusion of biodiversity in EISs using a systematic approach based on scientific papers, CBD, and a survey with 43 EIA practitioners from universities, government agencies, environmental consulting companies, business segment, and the third sector. The guidelines comprise 60 indicators arranged into eight categories about the project's characteristics, methods, baseline, impact assessment, alternatives, as well as mitigation, compensation, and monitoring measures. The guidelines also include the levels of biological diversity (ecosystem, species, and genetic diversity), the three elements of biodiversity (composition, structure, and key processes), and the main anthropogenic threats. Thus, the guidelines represent a methodological contribution to EIA that could support decision making and future systematic reviews of EISs. |