| 潍坊、花园口土样中土著大豆根瘤菌的遗传多样性研究 |
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| 引用本文： | 杨江科,周俊初. 潍坊、花园口土样中土著大豆根瘤菌的遗传多样性研究[J]. 应用与环境生物学报, 2000, 6(3): 259-262 |
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| 作者姓名： | 杨江科 周俊初 |
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| 作者单位： | 农业部农业微生物重点实验室,华中农业大学,武汉,430070 |
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| 基金项目： | 受欧盟INCO-DCERBIC970 191课题经费资助 |
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| 摘 要： | 采用１６Ｓ－２３Ｓ ｒＤＮＡ间隔区段（ＩＧＳ）ＲＦＬＰ分析和ＰＡＰＤ分析技术，分别对分离自山东潍坊和河南郑州花园口的２４株土著大豆根瘤菌进行２多样性分析。结果一 ７０％的相似性水平上，依ＩＧＳ－ＲＦＬＰ分析可将供试菌株分为１０群，依ＲＡＰＤ分析可将供试菌株分为８群。综合两种分析技术在５０５的相似性水平上将供试菌株分为潍坊群和花园口群。 在系统发育和分子进行上有明显的地理分隔作用。
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| 关 键 词： | 土著大豆根瘤菌 遗传多样性 系统发育 地理分隔 |
GENETIC DIVERSITY OF INDIGENOUS SOYBEAN RHIZOBIA ISOLATED FROM SOILS IN WEIFANG AND HUAYUANKOU |
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| YANG Jiangke,ZHOU Junchu. GENETIC DIVERSITY OF INDIGENOUS SOYBEAN RHIZOBIA ISOLATED FROM SOILS IN WEIFANG AND HUAYUANKOU[J]. Chinese Journal of Applied and Environmental Biology, 2000, 6(3): 259-262 |
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| Authors: | YANG Jiangke ZHOU Junchu |
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| Abstract: | 16S-23SrDNA intergenic spacer(IGS) RFLP and randomly amplified polymorphic DNA(RAPD) were used toanalyze the genetic diversity of 24 indigents soybean rhizobial strains isolated fromsoils in Weifang and Huayuankou. The results showed that all strains tested could bedivided into 10 or 8 small clusters at the similarity level of 70% by 16S-23S rDNAIGS-RFLP and RAPD, respectively. According the combined results of IGS-RFLP and RAPD, allstrains tested could be divided into Weifang and Huayuankou clusters at the similaritylevel of 50% or so, which showed geographical insulation in systematic development andmolecular evolution. The difference of results between IGS-RFLP and RAPD demonstrated thattotal DNA and the IGS sequence had heterogeneous characteristic and IGS-RFLP was moresensitive for detecting genetic diversity of soybean rhizobia. Fig 6 Tab 2 Ref 8 |
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| Keywords: | indigenous rhizobia 16S 23S rDNA IGS RFLP RAPD |
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