细菌细胞中类异戊二烯醌的分析

类异戊二烯醌是细菌质膜的成分,在电子传递和氧化磷酸化中起重要作用.甲基萘醌和泛醌是其中较为重要的两类化合物.这两种化合物的分析鉴定是研究细菌细胞中类异戊二烯醌组成构象的前提,在细菌化学分类的研究中具有十分重要的意义.本文参考了有关细菌类异戊二烯醌分析的文献,综述了甲基萘醌和泛醌的分析方法,其中包括:液-液萃取、薄层色谱(TLC)、高效液相色谱(HPLC)、紫外吸收光谱(UV)、质谱(MS)的定性鉴定及HPLC的定量分析.

ANALYSIS OF BACTERIAL ISOPRENOID QUINONES

Isoprenoid Quinones are constituents of bacterial plasma membranes and play important roles in electron transport and oxidative phosphorylation. The two major types of bacterial isoprenoid quinones are menaquinones and ubiquinones. The analysis of menaquinones and ubiquinones is the prerequisite of study of bacterial isoprenoid quinones profiles, which plays an important part in bacterial chemotaxonomic study. In this paper analysis methods of menaquinones and ubiquinones were reveiwed. It includes liquid-liquid extraction, qualitative identification by thin layer chromatography (TLC), high performance liquid chromatography (HPLC), ultraviolet spectroscopy (UV) and mass spectrometry (MS), and accurade quantitative analysis by HPLC as well.