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Bioconcentration of nonylphenol in fathead minnows (Pimephales promelas) |
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| Authors: | Snyder S A Keith T L Pierens S L Snyder E M Giesy J P |
| Institution: | Department of Zoology, Institute of Environmental Toxicology, 218C National Food Safety and Toxicology Center, Michigan State University, East Lansing, MI 48824-1311, USA |
| Abstract: | Bioconcentration of p-nonylphenol (NP) by fathead minnows was determined under laboratory conditions. Fish were exposed continuously for 42 days to 0.33, 0.93 and 2.36 μgNP/l in a flow-through system. NP was Soxhlet extracted from whole fish homogenates with dichloromethane (DCM). The resulting extract was concentrated and bulk lipids removed by gel permeation and silica-gel chromatography. Compounds were identified and quantified by reverse-phase high-pressure liquid chromatography (RP-HPLC) with fluorescence detection. Mass spectrometry was used for verification of peak assignments. Bioconcentration factors (BCFs) ranged from 245 to 380. |
| Keywords: | Bioaccumulation Alkylphenol Surfactant Bioconcentration factor |
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