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Chorionic villus metaphase chromosomes and interphase nuclei analysed by chromosomal in situ suppression (CISS) hybridization |
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| Authors: | Martin Klever Caspar J Grond-Ginsbach Hans-Dieter Hager Traute M Schroeder-Kurth |
| Institution: | Institut für Humangenetik und Anthropologie, Im Neuenheimer Feld 328, 69 Heidelberg, Germany |
| Abstract: | Metaphase chromosomes and interphase nuclei of chorionic villus samples (CVS) in five cases were studied after treatment with trypsin and post-fixation in formaldehyde by chromosomal in situ suppression (CISS) hybridization. Our modified protocol enables the use of in situ hybridization. techniques on CVS preparations after 42 h ofculture. A balanced translocation and trisomy 13 were identified with the aid of CISS hybridization. |
| Keywords: | First-trimester prenatal cytogenetical diagnosis Chromosomal in situ suppression (CISS) hybridization Interphase cytogenetics |