Human chorionic β-mannosidase: Comparison with β-mannosidase from human cultured fibroblasts |
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| Authors: | N A Petushkova T S Ivleva Ya M Vozniy |
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| Institution: | Institute of Biological and Medical Chemistry, Russian Academy of Medical Sciences, Pogodin Street, 10, Moscow, 119832, Russia |
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| Abstract: | The conditions for assay of β-mannosidase activity in human chorionic villi (CV) were studied using the fluorogenic substrate 4-methylumbelliferyl-β-D-mannopyranoside. A comparison of the biochemical properties of the CV β-mannosidase with those of the enzyme from human cultured fibroblasts showed their similarity. Like the enzyme from skin fibroblasts, the CV β-mannosidase had rather high activity. Both enzymes had virtually the same pH optimum (4.2-4.7) and Km value. The data presented suggest that chorion biopsy specimens can be used for prenatal determination of β-mannosidase activity at an early stage of development. |
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| Keywords: | β-mannosidase Chorionic villi Early prenatal diagnosis Cultured fibroblasts |
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