Use of dimethyl sulfoxide as solubilization agent in the detection of 2,3,7,8-TCDD by radioimmunoassay |
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| Authors: | J.P. Sherry J.W. ApSimon T.L. Collier P.W. Albro |
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| Affiliation: | J.P. Sherry*, J.W. ApSimon, T.L. Collier,P.W. Albro |
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| Abstract: | Immunoassays are potentially valuable tools for use in screening environmental samples for a broad range of contaminants, such as polychlorinated dibenzo-p-dioxins (PCDDs). The performance of the radioimmunoassay (RIA) for PCDDs was characterized using 4 solubilization systems: Cutscum, Triton, horse serum, and dimethyl sulfoxide (DMSO). The DMSO based assay appeared to perform best at low 2,3,7,8-TCDD levels. The effects of assay incubation time and hapten storage conditions on the DMSO based assay were assessed. The separation of bound from unbound radioactivity was accelerated without adversely affecting assay performance. Further assay development through the use of an alternative labelled hapten is considered. |
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| Keywords: | Radioimmunoassay polychlorinated dibenzo-p-dioxins screening 125I labelling sensitivity detection limit precision solubilization |
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