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Nanodiagnostics for plant pathogens
Authors:Prem Lal Kashyap  Sudheer Kumar  Alok K Srivastava
Institution:1.ICAR-Indian Institute of Wheat and Barley Research (IIWBR),Shimla,India;2.ICAR-Indian Institute of Wheat and Barley Research (IIWBR),Karnal,India;3.ICAR-National Bureau of Agriculturally Important Microorganisms,Mau,India
Abstract:Rapid detection technologies with high sensitivity and selectivity for plant pathogens are essential to prevent disease spread and minimize losses to assure optimal productivity and food security. Traditional laboratory techniques such as microscopy and culture are time-consuming, labour intensive and require complex sample handling. Immunological and molecular techniques have advanced but have some issues related to rapidity, signal strength and instrumentation. The integration of immunological and molecular diagnostics with nanotechnology systems offers an option where all detection steps can be accommodated on a portable miniaturized device for rapid and accurate detection of plant pathogens. The sensitive nature of functionalized nanoparticles can be used to design phytopathogen detection devices with smart sensing capabilities for field use. This review summarizes the current status and future prospects of nanotechnology for detection and diagnosis of plant pathogens.
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