丁草胺对镉胁迫条件下水稻生长、镉积累及活性氧代谢的影响

以2个水稻品种(秀水110和秀水11)为材料,通过水培试验和土培试验研究了除草剂丁草胺对Cd2 胁迫条件下水稻植株生长、Cd2 积累和活性氧代谢的影响.研究结果表明.水培条件下,丁草胺浓度的增加显著地加剧了Cd2 胁迫条件下水稻生长抑制程度,进一步导致叶绿素含量显著下降,促进了2个品种水稻植株对Cd2 的吸收,引起水稻叶片的O-2产生速率、H202和MDA含量增加.且显著改变了SOD、CAT和G-POD活性;同条件下丁草胺浓度的增加使水稻叶片SOD/O-2、SOD/G-POD、SOD/CAT、CAT/H2O2和G-POD/H2O2的比值均发生了偏离;推测丁草胺加剧了水稻Cd2 胁迫时植株活性氧代谢不平衡过程.土培条件下,丁草胺对水稻糙米中Cd2 积累量也具有显著促进作用.

Effects of butachlor on the growth, cadmium accumulation and oxidative metabolism of rice under Cd2+stress

The effects of butachlor on the oxidative metabolism,accumulation of cadmium and growth of cadmium-treated rice seedlings(Xiushui110 and Xiushui11)were studied under hydroponic and soil culture conditions.For hydroponically grown rice,butachlor aggravated the cadmium-induced inhibition of growth.With increasing concentration of butachlor,the chlorophyll content of leaves decreased dramatically and the cadmium accumulation of plants increased significantly.At the same time,the rate of O₂ generation,and content of H₂O₂ and MDA in the leaves were obviously enhanced.With the increase of butachlor concentration,the activity of SOD,CATand G-POD of leaves and the ratios of SOD/O₂,SOD/G-POD,SOD/CAT,CAT/H₂O₂ and G-POD/H₂O₂ also changed considerably.It is hypothesized that butachlor disturbs the equilibrium between active oxygen species and antioxidant enzymes.In soil-grown brown rice,the results indicated that butachlor significantly increased the cadmium content.