| 转基因烟草中赖氨酸含量的提高 |
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| 引用本文： | 王逸群,郑金贵,谢宝贵.转基因烟草中赖氨酸含量的提高[J].应用与环境生物学报,2005,11(1):32-35. |
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| 作者姓名： | 王逸群 郑金贵 谢宝贵 |
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| 作者单位： | 1. 福建师范大学生物工程学院,福州,350007;福建农林大学生物技术中心,福州,350002
2. 福建农林大学生物技术中心,福州,350002 |
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| 基金项目： | 国家自然科学基金 (30070467)，福建省科技厅科技项目(2001Z012)，福建省博士后基金项目资助~~ |
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| 摘 要： | 采用叶盘法将高赖氨酸蛋白基因导入到烟草中.GUS组织化学染色、PCR扩增、Southernblot分析表明, 该基因已经整合到烟草基因组中.测定 10株转基因烟草叶片赖氨酸含量,大部分植株有着明显的提高,最高幅度达到了49. 89%. 图 6参 11
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| 关 键 词： | 转基因烟草 赖氨酸含量 提高 |
| 修稿时间： | 2004年1月4日 |
IMPROVEMENT OF LYSINE CONTENT IN TRANSGENIC TOBACCO |
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| Wang Yiqun,ZHENG Jingui,XIE Baogui.IMPROVEMENT OF LYSINE CONTENT IN TRANSGENIC TOBACCO[J].Chinese Journal of Applied and Environmental Biology,2005,11(1):32-35. |
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| Authors: | Wang Yiqun ZHENG Jingui XIE Baogui |
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| Abstract: | In this experiment, the lysine-rich protein gene was transformed into tobacco by leaf disc via Agrobacterium tumefaciens LBA4404 as a vehicle. Histochemical staining for GUS activity, PCR and Southern blot demonstrated that lys was integrated into the genome of tobacco plants. Data analysis showed that lysine content in most of 10 transgenic plants was obviously improved and the content in 1 transgenic plant was increased by 49.89%. Fig 6, Ref 11 |
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| Keywords: | transgenic tobacco lysine content improvement |
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