| 杆状病毒包涵体蛋白的共同抗原性 |
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| 引用本文： | 刘彩云,刘金瑾,汤显春,张忠信,彭辉银,孙修炼. 杆状病毒包涵体蛋白的共同抗原性[J]. 应用与环境生物学报, 2008, 14(6). DOI: 10.3724/SP.J.1145.2008.00787 |
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| 作者姓名： | 刘彩云 刘金瑾 汤显春 张忠信 彭辉银 孙修炼 |
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| 作者单位： | 中国科学院武汉病毒研究所病毒学国家重点实验室,武汉,430071 |
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| 基金项目： | 国家"863"计划项目 ， 中国科学院知识创新工程重要方向项目资助 Supported by the"863"Project from Ministry of Science& Technology of China ， the Knowledge Innovation Program of the Chinese Academy of Sciences |
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| 摘 要： | 在比对已公布的37种杆状病毒包涵体蛋白氨基酸序列的基础上,选定棉铃虫核型多角体病毒(HaNPV)多角体蛋白的两个高度保守多肽(54-113aa和206-245aa)制备多克隆抗体,用Western blot法检测这两个高度保守多肽与多种杆状病毒包涵体蛋白之间的血清学关系.结果表明,HaNPV多角体蛋白的两个多肽的抗体与14种核型多角体病毒的多角体蛋白和5种颗粒体病毒的颗粒体蛋白均有明显的杂交信号,表明杆状病毒的包涵体蛋白之间具有共同的抗原决定簇.根据杆状病毒包涵体蛋白之间的这种血清学关系,进一步讨论了利用免疫金试纸技术检测病毒杀虫剂中包涵体含量的可行性.
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| 关 键 词： | 杆状病毒 棉铃虫核型多角体病毒 包涵体蛋白 保守多肽 共同抗原性 |
Occlusion Body Proteins of Baculoviruses Sharing Common Epitopes |
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| LIU Caiyun,LIU Jinjin,TANG Xianchun,ZHANG Zhongxin,PENG Huiyin,SUN Xiulian. Occlusion Body Proteins of Baculoviruses Sharing Common Epitopes[J]. Chinese Journal of Applied and Environmental Biology, 2008, 14(6). DOI: 10.3724/SP.J.1145.2008.00787 |
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| Authors: | LIU Caiyun LIU Jinjin TANG Xianchun ZHANG Zhongxin PENG Huiyin SUN Xiulian |
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| Affiliation: | LIU Caiyun,LIU Jinjin,TANG Xianchun,ZHANG Zhongxin,PENG Huiyin & SUN Xiulian(State Key Laboratory of Virology,Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan 430071,China) |
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| Abstract: | Based on the alignment of the published amino acid sequences of occlusion body proteins of 37 baculoviruses, two highly conversed regions among the proteins were found. The polyclonal antibodies against the two highly conversed peptides (54-113aa and 206-245aa) of polyhedrin of Heliocoverpa armigera Nucleopolyhedrovirus (HaNPV) were raised. The serological relationships between the two peptides and the occlusion body proteins of other baculovirus were investigated by using Western blotting. The results reve... |
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| Keywords: | Western blot |
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