Noncovalent bonds are key mechanisms for the cohesion of barnacle (Balanus crenatus) adhesive proteins |
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Authors: | Maja Wiegemann Thomas Kowalik Andreas Hartwig |
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Affiliation: | (1) IFAM Bonding Technology and Surfaces Department, Fraunhofer Institute for Manufacturing Engineering and Applied Materials, Wiener Str. 12, 28359 Bremen, Germany;(2) Present address: Alfred-Wegener-Institut, Am Handelshafen 12, 27570 Bremerhaven, Germany |
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Abstract: | Cement of the barnacle species, Balanus crenatus, was isolated and separated by SDS-PAGE. Reductive and nonreductive conditions yielded the same result of six major proteins (in the range up to 100 kDa) occurring as two triplets. A third triplet of lower molecular mass was faintly visible. The presence of a reductive agent had only little impact on the solubility of B. crenatus cement. The strong effect of the denaturing conditions (the presence of SDS and the incubation at elevated temperature) on the solubility of the barnacle cement and the absence of disulfide signals in Raman spectra suggest a complex structure of the barnacle cement noncovalently bound. It can be speculated that this protein complex in its quaternary structure is formed by small subunits and is equivalent to the globular cement structures previously detected. |
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