Comparative Toxicity of Copper and Zinc to Isolated Eel Hepatocytes |
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Authors: | Deng-Fwu Hwang Jui-Fen Lin Sen-Shyong Jeng |
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Institution: | Department of Marine Food Science , National Taiwan Ocean University , Keelung 202, Taiwan, R.O.C. |
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Abstract: | Attempts were made to elucidate lethal responses of hepatocytes of Japanese eel (Anguilla japonica) exposed to Krebs-HEPES buffer at different concentrations of Cu++ and Zn++. It was found that the 2-hr LC50 value of Cu++ and Zn++ on eel hepatocytes was 490 and 1576 μM for Cu++ and Zn++ in single solution, and 1734 and 5200 μM in mixed solution. the toxic effects of Cu++ and Zn++ on eel hepatocytes were characteristic of antagonistic interaction. After 2-hr exposure, the amount of accumulated metal on the hepatocytes and the mortality of hepatocytes were increased with increasing metal concentration in Krebs-HEPES buffer. the accumulated amount of both metals was significantly decreased when the eel hepatocytes were exposed to mixed metals than to single metal. |
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Keywords: | Copper zinc Japanese eel hepatocytes toxicity LC50 |
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