In vitro cytotoxicity of multi-wall carbon nanotubes on human cell lines |
| |
| Authors: | Anreddy Rama Narsimha Reddy Devarakonda Rama Krishna Vurimindi Himabindu |
| |
| Institution: | 1. Department of Pharmacology and Toxicology , Kakatiya University , Warangal 506009, India;2. Department of Pharmacology and Toxicology , Kakatiya University , Warangal 506009, India;3. Covidien Pharmaceuticals , 675 McDonnell Boulevard, St. Louis, Hazelwood, MO 63042, USA;4. Centre for Environment , Institute of Science and Technology , JNTU, Hyderabad 500085, India |
| |
| Abstract: | The aim of this study was to evaluate the in vitro toxicity of two multi-wall carbon nanotubes on four different cell lines: human alveolar epithelial (A549) cells, hepatocytes (Hep 3B cells), human embryonic kidney cells, and intestinal (P407 cells) cells. The adverse effects of carbon nanoparticles were analyzed after 24 h incubation with different cell lines using the trypan blue dye exclusion method. Incubation of carbon nanotubes with different cells produced a concentration-dependent inhibition of growth of the cells. The TC50 or IC50 values (toxic concentration 50, i.e., concentration of particles inducing 50% cell mortality) of two nanoparticles were (1) found to be in the range 23.5–30.5 µg mL?1, and (2) less than that of quartz (known toxic agent, 28.8–66.9 µg mL?1), indicating the greater cytotoxic effect of carbon nanoparticles than quartz particles. |
| |
| Keywords: | carbon nanotubes MWCNTs A549 Hep 3B HEK P407 toxicity in vitro |
|
|
