Solid-Phase Microextraction As A Tool for Studying Volatile Compounds in Frog Skin |
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| Authors: | Benjamin P. Smith Claudia Alcaraz Zini Janusz Pawliszyn Michael J. Tyler Yoji Hayasaka Brian Williams |
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| Affiliation: | 1. Department of Environmental Biology , University of Adelaide , South Australia, Australia;2. Department of Soil and Water , University of Adelaide , South Australia, Australia;3. Department of Soil and Water , University of Adelaide , Adelaide, South Australia, Australia , 5005 Phone: +618 8303 7385 Fax: +618 8303 7385 E-mail: benjamin.smith@adelaide.edu.au;4. Department of Chemistry , University of Waterloo , Ontario, Canada;5. Instituto de Química, Universidade Federal Do Rio Grande Do Sul , Brazil;6. Department of Chemistry , University of Waterloo , Ontario, Canada;7. Department of Environmental Biology , University of Adelaide , South Australia, Australia;8. Australian Wine Research Institute , South Australia, Australia;9. Department of Soil and Water , University of Adelaide , South Australia, Australia |
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| Abstract: | Solid-phase microextraction (SPME) is an effective technique for studying frog volatile secretions. Its primary advantage is in its application to sampling live animals. the ability to sample an organism over an extended period allows changes in an individual's chemical signature to be determined. the presence of eucalyptol in the skin secretion of Ewing's tree frog, Litoria ewingi, was used to assess the effectiveness of SPME in sampling frog volatiles. Rapid sample times coupled with the polydimethylsiloxane/divinylbenzene (PDMS/DVB) fibre provided the best signal/noise ratio for the majority of frog volatiles analysed, and importantly resulted in the least amount of stress to the animals involved. |
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| Keywords: | Solid-phase microextraction Frog volatile secretions |
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