A rapid determination of histamine and 3-methylhistamine in human plasma by high performance liquid chromatography |
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Authors: | Mohammadreza Ghandforoush-Sattari Siminozar Mashayekhi |
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Institution: | 1. Faculty of Pharmacy , Department of Pharmacology and Toxicology , Tabriz University of Medical Sciences, Tabriz, Iran mrgsuk@yahoo.com;3. Haematology and Oncology Research Centre , Tabriz University of Medical Sciences , Tabriz, Iran |
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Abstract: | Histamine plays an important pathophysiological role in allergy, inflammation, gastric acid secretion, microcirculation and neurotransmission. 3-Methylhistamine is a prominent metabolite of histamine. Different methods for determination of histamine in biological fluids have been developed. In the present study, a simple, simultaneous determination of histamine and 3-methylhistamine by HPLC (precolumn derivatization with fluorescamine) was developed in human plasma using fluorescence detection with 1-methylhistamine as the internal standard. Linear regression analysis of the ratios of the concentrations of histamine and 3-methylhistamine (X) against peak height ratios (Y) yielded the following: y = 0.0073x ? 0.0096 (R 2 = 0.990) and y = 0.0077x ? 0.0111 (R 2 = 0.989). In conclusion, it was possible to detect histamine and 3-methylhistamine below 5 ng mL?1 in 1 mL plasma. |
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Keywords: | histamine 3-methylhistamine 1-methylhistamine O-phthalaldehyde HPLC fluorescamine |
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