Effect of acute whole-body proton irradiation on hematological and intestinal variables in mice |
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Authors: | Ji-Young Shim Ji-Yeon Ahn Jae Youn Yi Chang-Mo Kang Yeon-Sook Yun |
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Affiliation: | 1. Laboratory of Radiation Sensitization and Protection , 215-4 Gongneung-dong, Nowon-gu, Korea Institute of Radiological and Medical Sciences , Seoul 136-706, Korea;2. Laboratory of Modulation of Radiobiological Responses, 215-4 Gongneung-dong, Nowon-gu , Korea Institute of Radiological and Medical Sciences , Seoul 136-706, Korea;3. Laboratory of Radiation Cytogenetics and Epidemiology, 215-4 Gongneung-dong, Nowon-gu , Korea Institute of Radiological and Medical Sciences , Seoul 136-706, Korea |
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Abstract: | Female C57BL/6 mice were exposed to a single dose of protons or 60Co gamma rays at 20 mGy and 2 Gy, which represent the recommended limit for annual occupational exposure and the daily fractional dose for patients undergoing radiotherapy, respectively. A control group was not exposed to radiation. The number of peripheral blood, bone marrow, and spleen cells was assessed 24 h after radiation to investigate the marrow suppression. The number of lymphocytes and neutrophils in peripheral blood was significantly lower in mice exposed to the higher dose of radiation than in the nonirradiated mice. However, at a low dose of radiation, there were no discernable hematological effects. The frequency of micronuclei in bone marrow cells was increased only after the high dose of gamma radiation. Radiation-induced intestinal apoptosis was measured using terminal deoxynucleotide transferase labeling assay. The magnitude of damage was greater after gamma irradiation than after proton irradiation at the same dose. Among the systemic biological end point to predict whole body radiation effect, the apoptosis of intestinal crypts may be the most sensitive parameter to provide information even at low dose of radiation. |
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Keywords: | proton gamma irradiation hematology intestine micronuclei |
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