Toxic effect of microcystin-LR on blood vessel development |
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| Authors: | Qilong Wang Guoliang Chen Huihui Du Linping Wang Dongqin Guo |
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| Institution: | 1. College of Biology and Food Engineering, Chongqing Three Gorges University, Chongqing, PR China;2. Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, PR China;3. Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, PR China |
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| Abstract: | AbstractThe effect of microcystin-LR (MC-LR) at 50–1000?nmol/L on blood vessel development of zebrafish embryos has been explored. Upon exposure 72-h-post-fertilization (hpf), the hatching rate was significantly reduced and malformation and fatality rates were increased in a dose-dependent manner. MC-LR induced cell cycle arrest in the G0/G1-phase and damaged DNA structure in a dose-dependent manner. In human umbilical vein endothelial cells (HUVECs), MC-LR led to decreased activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) and increased lipid peroxidation. These findings indicated that MC-LR could cause oxidative DNA damage, which mediated cell cycle arrest and inhibited cell proliferation afterwards, and retarded the blood vessel development. |
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| Keywords: | Blood vessel development human umbilical vein endothelial cell microcystin-LR toxicity effect zebrafish |
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