Effects of lead on calmodulin content,proliferation and interleukin-2 in lymphocytes of mice |
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| Authors: | Sun Li Liang Guo-Qing Jian Su-Jun Yuan Hong |
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| Institution: | 1. The Affiliated Children's Hospital, Medical School, Zhejiang University , Hangzhou , 310003, Zhejiang , P.R. China;2. Laboratory Animal Science , Medical School, Hangzhou Normal University , No. 16, Xuelin Road, Hangzhou 310036 , Zhejiang , P.R. China;3. Laboratory Animal Science , Medical School, Hangzhou Normal University , No. 16, Xuelin Road, Hangzhou 310036 , Zhejiang , P.R. China;4. Laboratory Animal Science , Medical School, Hangzhou Normal University , No. 16, Xuelin Road, Hangzhou 310036 , Zhejiang , P.R. China |
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| Abstract: | The mechanism inderlying lead (Pb)-induced toxicity was presumed to involve interaction between Pb2+ and calcium (Ca2+) associated with calmodulin-dependent systems. The aim of this study was to (1) examine the hypothesis that Pb alters calmodulin content and (2) assess the effects of Pb on proliferation and interleukin-2 (IL-2) levels in lymphocytes. The influence of Pb on calmodulin content in mouse lymphocytes was assessed by western blot, while the proliferation of lymphocytes was studied using MTT test, and IL-2 level by ELISA assay. The results showed that 100?µM PbCl2 decreased calmodulin content, phytohemagglutinin (PHA)-induced proliferation, and IL-2 levels, but a lower concentration (1?µM PbCl2) did not appear to be effective. Evidence indicates that IL-2 levels and lymphocyte proliferation might not be calmodulin content dependent. Our data suggested that Pb2+-induced toxicity was likely to be complex with multiple factors involved, including calmodulin, IL-2, and proliferation of lymphocytes. |
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| Keywords: | lead lymphocyte calmodulin cell proliferation interleukin-2 |
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