Inhibitory effect of arachidonic acid on venom acetylcholinesterase |
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| Authors: | Mushtaq Ahmed Nadia Latif Rahmat Ali Khan Akhlaq Ahmad Gustavo Thomé Maria R.C. Schetinger |
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| Affiliation: | 1. Department of Biotechnology , University of Science and Technology , Khyber Pakhtunkhwa, Bannu, Pakistan mushtaq213@yahoo.com ahmedbiochem@yahoo.com;3. Departamento de Bioquímica Toxicologia , Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria , 97105-900 Santa Maria, RS, Brazil;4. Department of Biotechnology , University of Science and Technology , Khyber Pakhtunkhwa, Bannu, Pakistan |
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| Abstract: | The venom of krait (Bungarus sindanus), an Elapidae snake, contains high level of acetylcholinesterase (AChE) activity. The effect of arachidonic acid on krait (Bungarus sindanus) venom AChE (acetylcholine hydrolase, EC 3.1.1.7) was studied using kinetic methods. This study evaluated for the first time whether arachidonic acid acts as potential AChE inhibitor of krait venom. Kinetic studies, using Line-Weaver Burk and Dixon plots, indicated that the inhibition produced by arachidonic acid was non-competitive, i.e., both km and V max fell with increase in the concentration of arachidonic acid. The concentration of arachidonic acid required to inhibit 50% of enzymatic activity was found to be 1.9?µM while the inhibitory constant (Ki ) was 1.4?µM. The present results suggest that essential fatty acid (arachidonic acid) may also be used as an inhibitor of snake venom AChE. |
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| Keywords: | arachidonic acid ninhibition acetylcholinesterase snake venom |
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