Novel method for in situ visualization of polycyclic aromatic hydrocarbons in mangrove plants |
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| Authors: | Ping Wang Yong Zhang |
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| Institution: | 1. School of Environmental Science and Public Health, Wenzhou Medical College , Wenzhou, China;2. State Key Laboratory of Marine Environmental Science, Xiamen University , Xiamen, China;3. Environmental Science Research Center, Xiamen University , Xiamen, China |
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| Abstract: | Two-photon laser confocal scanning microscopy (TPLCSM) was first used to visualize the uptake and movement of naphthalene (NAP), phenanthrene (PHE), and pyrene (PYR), from separately contaminated hydroponics solutions into living Kandelia candel (L.) Druce seedlings. With this non-destructive and non-chemical extraction technique, the experimental results revealed that three types of polycyclic aromatic hydrocarbons (PAH) were observed moving into the K. candel and its translocation to radicle, hypocotyl and leaf, and the transmission rates of these PAH in the K. candel were in the order of NAP?>?PHE?>?PYR. Data demonstrate the manner in which PAH enter, transport, and distribute within the K. candel, and provided us some valuable information on uptake and translocation mechanism of PAH. These findings may help to optimize the phytoremediation strategies of PAH in mangrove wetlands. |
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| Keywords: | Kandelia candel (L ) Druce seedling PAHs two-photon laser confocal scanning microscopy |
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