A comparative study of microbial communities in soils amended by manures from pigs fed with organic versus synthetic feeds |
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Authors: | Xiao-Ming Lu Peng-Long Lu Peng-Zhen Lu Hui Zhang |
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Institution: | 1. Agriculture and Biotechnology Department, Institute for Eco-Environmental Sciences, Wenzhou Vocational College of Science &2. Technology, Wenzhou, People's Republic of China2009533@wzvcst.edu.cn;4. Advertising Agency, Wenzhou Transportation Group, Wenzhou, People's Republic of China;5. Department of Civil Engineering, Faculty of Civil Engineering and Architecture, Zhejiang University of Technology, Hangzhou, People's Republic of China;6. Technology, Wenzhou, People's Republic of China |
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Abstract: | We applied pyrosequencing to determine bacterial communities in soil samples taken from test fields amended by manures of pigs fed with green feeds (GPMS), synthetic feeds (APMS), and the reference field without swine manure amendment (NPMS), respectively. For each sample, 7050 effective sequences were selected and utilized to do the bacterial diversity and abundance analysis, respectively. In total, 2986, 2873, and 1602 operational taxonomic units were obtained at a 3% distance cut-off in GPMS, APMS, and NPMS, respectively. Bacterial phylotype richness in GPMS was higher than the other samples. NPMS had the least richness. The most dominant class in both GPMS and APMS is Betaproteobacteria, whereas Alphaproteobacteria is dominant in NPMS. Circa 7.5% sequences in GPMS were found to be affiliated with Burkholderiales order. Bacterial diversity and abundance in the soil varied with swine manure amendment, due to nutrient elements as well as toxic metals in swine manures. Compared with the soil amendment by manures from pigs bred with synthetic feeds, more bacterial diversity but less potentially pathogenic bacterial genera in the soil amended by manures of pigs fed with green feeds were found, which indicated that these manures were better land-applied organic fertilizers. |
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Keywords: | pyrosequencing soil bacterial community swine manure pig feed |
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