Analysis of genetic structure of blacklip abalone (Haliotis rubra) populations using RAPD, minisatellite and microsatellite markers |
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Authors: | B X Huang R Peakall P J Hanna |
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Institution: | (1) School of Biological and Chemical Sciences, Deakin University, Geelong, Victoria 3217, Australia Fax: 0061 (0)3 5227-2022 e-mail: pjh@deakin.edu.au, AU;(2) Division of Botany and Zoology, The Australian National University, Canberra, ACT 0200, Australia, AU |
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Abstract: | We investigated the utility of three polymerase chain-reaction (PCR)-based DNA molecular markers in analysing genetic structure
of the populations of the blacklip abalone Haliotis rubra (Leach) of Victoria, Australia. The DNA markers included 84 randomly amplified polymorphic DNA (RAPD) bands amplified using
six random primers, two minisatellites, GHR (putative growth-hormone-gene-repeat) and MIPR (putative mollusca-insulin-like
peptide-gene-repeat), and three microsatellites, RUBGT1 containing (GT)n repeats], RUBCA1 containing (CA)n repeats] and RUBGACA1 containing (GACA)n repeats]. All three types of DNA markers revealed significant subdivision in the H. rubra populations along the coastline. This is postulated as being related to the abalone's relatively short pelagic period and
limited dispersion. Further analysis revealed that a Point Cook population sampled from within the semi-enclosed Port Phillip
Bay was distinct from two other central zone populations (Apollo Bay and Cape Schanck). The genotypes of microsatellites indicated
excessive homozygotes across all the populations at all three microsatellite loci, and possible causes such as larval recruitment
pattern and asynchronous spawning are discussed. The excessive homozygotes recorded for the three microsatellite loci contrast
with those observed in the minisatellite loci GHR and MIPR, the heterozygosities of which were at Hardy–Weinberg equilibrium.
Received: 17 March 1999 / Accepted: 24 November 1999 |
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