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北京市大气细颗粒物的遗传和非遗传毒性研究
引用本文:张旻,付娟玲,何凌燕,王芸,胡敏,朱彤,周宗灿. 北京市大气细颗粒物的遗传和非遗传毒性研究[J]. 中国环境科学, 2003, 23(4): 0-0
作者姓名:张旻  付娟玲  何凌燕  王芸  胡敏  朱彤  周宗灿
作者单位:1.北京大学医学部公共卫生学院毒理学系 北京100083 ;2.北京大学环境学院 北京100871
摘    要:用胞质阻断微核试验与单细胞凝胶电泳法检测北京市大气PM2.5无机提取物与有机提取物对Balb/c 3T3细胞微核形成与DNA链断裂的影响;通过划痕染料示踪技术(SL/DT)观察PM2.5无机提取物与有机提取物对Balb/c3T3细胞间通讯的影响.发现PM2.5有机提取物可引起双核微核细胞率显著增加(P<0.01)及导致慧星细胞率和DNA迁移长度显著增加(P<0.01);PM2.5有机提取物引起细胞间通讯的抑制; PM2.5无机提取物未见明显毒作用.结果表明,PM2.5可引起染色体损伤和原发性DNA损伤,抑制细胞间通讯,其毒作用主要由其有机成分引起.

关 键 词:细颗粒物  双核微核  DNA链断裂  细胞间通讯  
文章编号:1000-6923(2003)04-0337-04
收稿时间:1900-01-01;
修稿时间:2002-12-20

Studies on the genetic and epigenetic toxicity of airborne fine particulate matter in Beijing
ZHANG Min,FU Juan-ling,HE Ling-Yan,WANG Yun,HU Min,ZHU Tong,ZHOU Zong-can. Studies on the genetic and epigenetic toxicity of airborne fine particulate matter in Beijing[J]. China Environmental Science, 2003, 23(4): 0-0
Authors:ZHANG Min  FU Juan-ling  HE Ling-Yan  WANG Yun  HU Min  ZHU Tong  ZHOU Zong-can
Affiliation:ZHANG Min1,FU Juan-ling1,HE Ling-Yan2,WANG Yun2,HU Min2,ZHU Tong2,ZHOU Zong-can1
Abstract:The effects of extracted organic components(EOC) and inorganic extracts of airborne particulate matter(PM2.5) in Beijing on micronucleus formation of Balb/c 3T3 cell and DNA strand breaks, were inspected using cytochalasin B-blocked test and single cell electrophoresis method; and their effects on gap junction intercellular communication (GJIC) of Balb/c 3T3 cells were observed through scrape-loading/dye transfer (SL/DT) technique. It was found that PM2.5 organic extracts increased significantly the rate of micronucleited binucleated cells (P<0.01), the frequency of comet cells and the distances of migration of DNA (P<0.01), and inhibited the GJIC; PM2.5 inorganic extracts did not have marked toxicity. Results demonstrated that PM2.5 could induce chromosome damage, primary DNA damage and inhibition of the GJIC; and its toxicity is mainly induced by its organic component.
Keywords:fine particulate matter  micronucleited binucleated cells  DNA strand breaks  gap junction intercellular communication (GJIC)
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