鄱阳湖老爷庙水域细菌群落组成分析

通过提取鄱阳湖老爷庙水域水体细菌总DNA, 构建细菌16SrRNA基因文库和序列测定等手段，以期揭示鄱阳湖老爷庙水域的细菌群落组成并探讨细菌多样性与湖泊水体理化参数之间的关系。研究结果认为：来自老爷庙水域的2006年10月（PN06 10）和2007年5月 (PN07 5) 的细菌文库中的180个阳性克隆产生了66个细菌分类操作单位（OTUs），这些细菌主要分属于：变形菌Alpha 纲，Beta 纲，Gamma 纲，Epsilon 纲，拟杆菌门，放线菌门，浮霉菌门，酸杆菌门和OP10等9大类型。2006年10月老爷庙水域的优势菌群为Beta 变形菌；2007年5月优势菌群为Gamma 变形菌和拟杆菌，表明鄱阳湖细菌组成在不同的季节显著不同。进一步系统发育分析表明：大部分细菌的最相似序列来源于淡水，湖泊和土壤的未培养细菌，且部分细菌与环境污染物降解有关

COMPOSITION OF BACTERIAL COMMUNITY IN THE LAO YE MIAO AREA OF LAKE POYANG

Composition of bacterial community was investigated through directly extracted the environmental DNA and sequencing cloned 16S rRNA from the water in the Lao Ye Miao area of Lake Poyang.The relationship between the bacterial community and water physical-chemical characteristics of Lake Poyang was discussed.From October in 2006 and May in 2007 clone libraries,one hundred and eighty bacterial clones were classified into sixty-six operational taxonomic units(OTUs),most of which were affiliated to Alpha-,Beta-,Gamma-,Epsilon-Proteobacteria,Bacteroidetes,Actinobacteria,Planctomycetes,OP10,and Acidobacteria.Beta-Proteobacteria was the most significant lineage in 2006 October library,while Gamma-Proteobacteria and Bacteroidetes were the most significant lineages in 2007 May library indicating that the bacterial community composition has seasonal characteristics.Phylogenetic analysis further showed that bacterial composition was highly similar with uncultured bacteria from freshwater and soil ecosystems,partial which related to degradation function.