中肋骨条藻高效溶藻菌FDHY-C3的分离鉴定及溶藻作用研究

从福建长乐海域的表层海水中分离获得一株对中肋骨条藻具有高效溶藻作用的溶藻菌FDHY-C3，通过形态学、生理生化及16S rDNA序列分析表明，菌株FDHY-C3属于交替单胞菌属(Alteromonas)。该菌株对常见的甲藻（dinoflagellate）、硅藻(diatom)以及针胞藻（raphidophyte）类的多种赤潮藻都有较高的溶藻作用，对中肋骨条藻溶藻效果尤为明显，72 h可达98.18%。溶藻菌FDHY-C3通过胞外分泌物间接溶藻；溶藻物质的溶藻特性不受反复冻融的影响，但是受酸碱性及温度的影响；溶藻活性物质具有被乙醇和乙酸乙酯沉淀的特性，为蛋白类物质，分子量为10～14 kD。

Isolation and identification of a high efficiency algicidal bacterium FDHY-C3 and algicidal characteristics on Skeletonema costatum

An algicidal bacterium (FDHY-C3) with high-efficiency algicidal activity was isolated from the surface water of Changle coastal area, Fujian province. The morphological, physiological, biochemical and 16S rDNA sequence analysis was performed and the results indicated that the strain FDHY-C3 belongs to the genus of Alternaria. This strain showed a broad algicidal activity on diatoms, dinoflagellates and raphidophytes. The highest algicidal rate was detected on Skeletonema costatum, the algicidal rate can reach 98.18% in 72 h. The algicidal compounds was detected in extracellular secretions of FDHY-C3 cells through an indirectly dissolve way on target algal cells. The algal-lytic compounds of FDHY-C3 were not affected by freezing, but were affected by acidity and alkalinity and high temperature. The compounds can be extracted by ethanol and ethyl acetate, and was estimated to be protease. The molecular weight of this compound was between 10 kD and 14 kD.