膜生物反应器对白斑综合症病毒的去除研究

研究了不同孔径的膜生物反应器对游离白斑综合症病毒(white spot syndrome virus,WSSV)的截留效果,对不同膜生物反应器的进水、上清液、出水进行荧光定量PCR检测.结果表明,0.45 μm孔径的膜组件对磷酸缓冲液中WSSV的去除量仅0.6 lg;0.22 μm孔径的膜对WSSV病毒的去除量为1.18 lg;0.1 μm孔径的膜对WSSV的去除量为5.5 lg.进一步研究表明,在养殖废水中添加游离的WSSV时,随着时间的延长,膜组件的生物样物质对WSSV去除起了重要的作用;孔径为0.45 μm的膜生物反应器,对WSSV的去除量也达到5.35 lg;3种不同孔径膜生物反应器中运行12h后,对WSSV的去除率并没有显著差异.

Removal Efficiency of the White Spot Syndrome Virus in the Membrane Bioreactors

White spot syndrome virus removal was performed in three submerged membrane bioreactors with the different membrane pore sizes. Samples were sampled from the influent, effluent and supernatant fluid in each MBR and were detected by a Taqman-based real-time PCR technology. When these MBRs with a pore of 0.45 microm, 0.22 microm, and 0.1 microm were treated the virus of WSSV isolated from the phosphorous buffer saline, the removal rate of WSSV could be achieved 0.6 lg, 1.18 lg, and 5.5 lg, respectively. The removal rate of the membrane module with a pore size of 0.45 microm was lowest than those of the other membrane modules. When the MBRs with the different pore sizes were treated the domestic aquaculture wastewater containing the WSSV, the removal efficiency of WSSV in the MBRs with a pore size of 0.22, 0.45 microm was increasing while the filtration resistance increased. It was revealed that the biofilm accumulating on the surface of the membrane made a major contribution to WSSV removal. No significant difference on the removal efficiency of WSSV was found (p > 0.05) in the MBRs on hour 12, the membrane with a pore size of 0.45 microm demonstrated an almost complete removal of WSSV (up to 5.35 lg).