Biodegradation of N,N-bis(carboxymethyl)-L-glutamate and its utilization as sole source of carbon,nitrogen, and energy by a Rhizobium radiobacter strain in seawater |
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Authors: | CG van Ginkel R Geerts |
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Institution: | 1. Product Safety &2. Regulatory Affairs, AkzoNobel, Arnhem, The Netherlands |
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Abstract: | Biodegradation of N,N-bis(carboxymethyl)-L-glutamate and its utilization by micro-organisms in marine environments were studied in Organization of Economic Development and Cooperation (OECD) screening tests and with pure bacterial cultures. Biodegradation in excess of 60% was achieved in closed bottle tests in less than 60 days, suggesting complete degradation. The bacterial strain isolated from marine sandy sediment and utilizing N,N-bis(carboxymethyl)-L-glutamate as sole source of carbon, nitrogen, and energy was identified as Rhizobium radiobacter. The strain was capable to mineralize N,N-bis(carboxymethyl)-L-glutamate in seawater and in synthetic media containing 0–60 g L?1 sea salts. Growth and adaptation studies indicated that N-carboxymethyl-L-glutamate and L-glutamate are main metabolites. |
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Keywords: | L-glutamate-N N-diacetate chelating agent marine environment biodegradation Rhizobium radiobacter |
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