Isolation of Cr(VI) reducing bacteria from industrial e uents
and their potential use in bioremediation of
chromium containing wastewater |
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Authors: | Ahmed Zahoor and Abdul Rehman |
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Institution: | Department of Microbiology and Molecular Genetics, University of the Punjab, New Campus, Lahore 54590, Pakistan. E-mail: ravian499@yahoo.com and Department of Microbiology and Molecular Genetics, University of the Punjab, New Campus, Lahore 54590, Pakistan. |
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Abstract: | The present study was aimed to assess the ability of Bacillus sp. JDM-2-1 and Staphylococcus capitis to reduce hexavalent chromium
into its trivalent form. Bacillus sp. JDM-2-1 could tolerate Cr(VI) (4800 g/mL) and S. capitis could tolerate Cr(VI) (2800 g/mL).
Both organisms were able to resist Cd2+ (50 g/mL), Cu2+ (200 g/mL), Pb2+ (800 g/mL), Hg2+ (50 g/mL) and Ni2+ (4000 g/mL).
S. capitis resisted Zn2+ at 700 g/mL while Bacillus sp. JDM-2-1 only showed resistance up to 50 g/mL. Bacillus sp. JDM-2-1 and S.
capitis showed optimum growth at pH 6 and 7, respectively, while both bacteria showed optimum growth at 37°C. Bacillus sp. JDM-
2-1 and S. capitis could reduce 85% and 81% of hexavalent chromium from the medium after 96 h and were also capable of reducing
hexavalent chromium 86% and 89%, respectively, from the industrial e uents after 144 h. Cell free extracts of Bacillus sp. JDM-2-1
and S. capitis showed reduction of 83% and 70% at concentration of 10 g Cr(VI)/mL, respectively. The presence of an induced protein
having molecular weight around 25 kDa in the presence of chromium points out a possible role of this protein in chromium reduction.
The bacterial isolates can be exploited for the bioremediation of hexavalent chromium containing wastes, since they seem to have a
potential to reduce the toxic hexavalent form to its nontoxic trivalent form. |
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Keywords: | bioremediation Bacillus sp JDM-2-1 Staphylococcus capitis |
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