| 麻疯树种质资源遗传多样性的ISSR分析 |
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| 引用本文： | 何玮,郭亮,王岚,杨威,唐琳,陈放. 麻疯树种质资源遗传多样性的ISSR分析[J]. 应用与环境生物学报, 2007, 13(4): 466-470 |
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| 作者姓名： | 何玮 郭亮 王岚 杨威 唐琳 陈放 |
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| 作者单位： | 四川大学生命科学学院生物资源与生态环境教育部重点实验室,资源生物学及生物制药工程省重点实验室,成都,610064 |
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| 基金项目： | 国家自然科学基金;教育部科学技术研究重点项目;教育部科学技术研究项目 |
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| 摘 要： | 采用ISSR分子标记技术对大戟科属麻疯树9个自然居群的遗传多样性水平和遗传结构进行了研究.用10个引物对9个居群共135个样品进行了扩增,共获得169条清晰的扩增位点,其中多态性位点164个.POPGENE分析结果表明,麻疯树居群具有丰富的遗传多样水平(多态百分率PPB=97.04%,Neis遗传多样性H=0.2357,Shannon信息指数I=0.3760).AMOVA分析表明,9个自然居群间遗传多样性出现了较大的遗传分化(Gst=0.5398)可能与其有限的基因流(Nm=0.4667)和遗传漂变有关;而居群内有限的遗传分化可能是由于麻疯树自交、近交占主导方式的繁育方式有关.并进行了麻疯树居群的聚类分析.图3表4参19
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| 关 键 词： | 麻疯树 遗传多样性 遗传结构 育种 |
| 收稿时间： | 2007-04-16 |
| 修稿时间： | 2007-05-10 |
ISSR Analysis of Genetic Diversity of Jatropha curcas L. |
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| HE Wei,GUO Liang,WANG Lan,YANG Wei,TANG Lin,CHEN Fang. ISSR Analysis of Genetic Diversity of Jatropha curcas L.[J]. Chinese Journal of Applied and Environmental Biology, 2007, 13(4): 466-470 |
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| Authors: | HE Wei GUO Liang WANG Lan YANG Wei TANG Lin CHEN Fang |
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| Affiliation: | Key Laboratory of Bio-resources and Eco-environnwnt, Ministry of Education, Provincial Key Laboratory of Resources Biology and Bio-pharmaceutical Engineering, College of Life Sciences, Siehuan University, Chengdu 610064, China |
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| Abstract: | Using ISSR analysis, the genetic diversity and genetic structure of 9 natural populations of Jatropha curcas L. were invested. Ten ISSR primers generated highly reproducible and stable DNA fragments. 164 of 169 loci were polymorphic (PPB=97.04%), and POPGENE analysis result (H=0.235 7, I=0.376 0) suggested a high level of genetic variation of J. curcas among the different populations. Based on AMOVA analysis, more variations occurred among the populations (Gst=0.539 8) than within a population. The high genetic variation level among the populations could be caused by not only the limited gene flow (Nm=0.466 7), but also the genetic drift. On the other hand, relatively low variation within a population might be related to the reproductive style. Based on the dendrogram result, this paper provides some strategies for the breeding of J. curcas. Fig 3, Tab 4, Ref 19 |
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| Keywords: | ISSR |
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