PCR-DGGE研究沸石植生混凝土微生物群落结构

采用PCR-DGGE技术研究了滨水区和非滨水区沸石植生混凝土内部微生物群落结构,结果表明:沸石植生混凝土内部微生物多样性指数和物种丰度值均很高,滨水区和非滨水区植生混凝土内部微生物多样性指数与物种丰度总体持平,但是各部分微生物丰度差异明显.滨水区微生物丰度值根部5~10cm>根部10~15cm>沸石表面生物膜.非滨水区微生物丰度沸石表面生物膜>根部5~10cm>根部10~15cm.基因测序结果和系统发育树分析可知.滨水区和非滨水区以及沸石混凝土内各部分之间优势菌种各不相同,在滨水区,沸石表面微生物膜中优势菌种为丙酸杆菌、都柏林克罗诺杆菌和葡萄球菌属,根部5~10cm中优势菌种为黄杆菌和沙门氏菌属,根部10~15cm中优势菌种为慢生根瘤菌属;非滨水区,沸石表面微生物膜中优势菌种为芽孢杆菌和红假单胞菌属,根部5~10cm中优势菌种为沙门氏菌、微球菌亚目的Agromyces和酸杆菌属,根部10~15cm中优势菌种为沙门氏菌属.

Research on community composition in zeolite-vegetation-concrete by PCR-DGGE

The microbial community composition in zeolite-vegetation-concrete which was grown in waterfront and embankment was investigated in this study with PCR-DGGE technology. The results showed that: Shannon diversity index and species abundance value of microbial community in the system were high, and the community composition in each section of the zeolite-vegetation-concret were very different depending on the depth. The order of species abundance value growed in waterfront of zeolite-vegetation-concrete was as followed (from high to low): rhizosphere soil (depth:5~10cm)> rhizosphere soil (depth:10~15cm)>zeolite surface biofilm, however, the order of species abundance value growed in embankment was very different from the order of waterfront section, which was zeolite surface biofilm> rhizosphere soil (depth:5~10cm)>rhizosphere soil (depth:10~15cm). Phylogenetic analysis revealed that, the pre-dominant species in zeolite-vegetation-concrete were different in each section. In waterfront section, the pre-dominant species in zeolite surface biofilm were Propionibacterium sp., Cronobacter dublinensis and Staphylococcus epidermidis, then Flavobacterium sp. and Salmonella sp. were the major microbe in rhizosphere soil with depth of 5~10cm, and Bradyrhizobium sp. was found to be the pre-dominant bacteria in rhizosphere soil with depth of 10~15cm. In embankment section, the pre-dominant microbe in zeolite surface biofilm were Paenibacillus sp. and Rhodopseudomonas sp., Salmonella sp., Agromyces sp. and Acidobacteria sp. were the major microbe group in rhizosphere soil with depth of 5~10cm, and Salmonella sp. was found to be the pre-dominant bacteria in rhizosphere soil with depth of 10~15cm.