恩诺沙星和硫氰酸红霉素对铜绿微囊藻的毒性研究

研究了恩诺沙星、硫氰酸红霉素暴露对铜绿微囊藻生长和生理的影响.结果显示恩诺沙星、硫氰酸红霉素对铜绿微囊藻的生长有抑制作用,且96h-EC50分别为84.6,48.2mg/L;对铜绿微囊藻的叶绿素荧光和光合色素含量的影响一致,表现为浓度-效应关系;对可溶性蛋白含量的影响表现为低促高抑的现象;丙二醛含量随着抗生素浓度的增加显著增加.可见,恩诺沙星和硫氰酸红霉素能够阻碍铜绿微囊藻的光合作用,抑制可溶性蛋白的合成,从而影响铜绿微囊藻的正常生长.

Toxicity of enrofloxacin and erythromycin thiocyanate on Microcystis aeruginosa

The toxic effects of two antibiotics, enrofloxacin and erythromycin thiocyanate, on the growth and physiology of Microcystis aeruginosa are studied. The results show that both enrofloxacin and erythromycin thiocyanate inhibited the growth of M. aeruginosa, and their 96h-EC50values for M. aeruginosa were 84.6μg/L and 48.2μg/L, respectively. The chlorophyll fluorescence and photosynthetic pigment of Microcystis aeruginosa were also inhibited by the antibiotics, and the inhibition effects increased with the increasing concentrations of enrofloxacin and erythromycin thiocyanate. The soluble protein content of M. aeruginosa was promoted in the presence of low concentrations of antibiotics (￡0.02mg/L), and was inhibited by high levels of antibiotics (30.05mg/L). The content of lipid peroxidation (malondialdehyde, MDA) presented a strong positive correlation with the levels of antibiotics. The study indicated that enrofloxacin and erythromycin thiocyanate both hindered the photosynthesis process of M. aeruginosa and the synthesis of soluble protein, thus inhibiting the growth of M. aeruginosa.