| 滇池沉积物中微囊藻毒素的HPLC检测 |
| |
| 引用本文： | 樊洁,邓南圣,刘碧波,肖邦定.滇池沉积物中微囊藻毒素的HPLC检测[J].云南环境科学,2005,24(3):18-20. |
| |
| 作者姓名： | 樊洁 邓南圣 刘碧波 肖邦定 |
| |
| 作者单位： | 武汉大学资源与环境科学学院 湖北武汉430072
(樊洁，邓南圣)，中科院水生生物研究所 湖北武汉430072
(刘碧波)，中科院水生生物研究所 湖北武汉430072(肖邦定) |
| |
| 基金项目： | 科技部973项目(2002CB412308)，中科院重大项目(KZCX1-SW-12)，国家高技术研究863课题(2001AA641030)联合资助。 |
| |
| 摘 要： | 通过向滇池沉积物中加入一定量的微囊藻毒素配制成模拟样品,再用提取液提取样品中的毒素,用HPLC方法检测其含量。通过对不同提取方式、不同提取液和两种微囊藻毒素变体的提取效果进行比较,发现采用正丁醇∶甲醇∶水=1∶4∶15的提取液,用多次搅拌提取的方式,MC-RR和MC-LR的回收率比较好。按此方式对滇池沉积物样品进行检测,发现共表层沉积物中有少量藻毒素的存在。
|
| 关 键 词： | 微囊藻毒素 滇池沉积物 HPLC检测 |
| 文章编号： | 1006-947X（2005）03-0018-03 |
| 收稿时间： | 2005-04-14 |
| 修稿时间： | 2005年4月14日 |
Research on Monitoring the Microcystins in Sediments of Dianchi Lake by HPLC Method |
| |
| Fan Jie;Deng NaSheng;Liu BiBo;Xiao BangDing.Research on Monitoring the Microcystins in Sediments of Dianchi Lake by HPLC Method[J].Yunnan Environmental Science,2005,24(3):18-20. |
| |
| Authors: | Fan Jie;Deng NaSheng;Liu BiBo;Xiao BangDing |
| |
| Abstract: | Eutrophic lake like Dianchi lake, where water bloom is extensive, is still worthy of investigating about the microcystins in sediments. In this study, microcystins were spiked in sediments, extracted and then detected by HPLC. Different extracting methods, solvents and microcystin variants were tested. Thus the suitable method is set up according to our experiments. By this method we found that there is indeed small amount of microcystins existing in the sediments of Dianchi lake. |
| |
| Keywords: | mimicrocystins sediment of Dianchi lake HPLC |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
