| 用SSR标记检测同源四倍体与二倍体水稻的遗传差异 |
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| 引用本文： | 栾丽,孔繁伦,何涛,刘刚,涂升斌.用SSR标记检测同源四倍体与二倍体水稻的遗传差异[J].应用与环境生物学报,2004,10(5):556-558. |
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| 作者姓名： | 栾丽 孔繁伦 何涛 刘刚 涂升斌 |
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| 作者单位： | 1. 中国科学院成都生物研究所,成都,610041;中国科学院研究生院,北京,100039
2. 中国科学院成都生物研究所,成都,610041 |
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| 基金项目： | 四川省应用基础研究项目资助~~ |
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| 摘 要： | 随机选用分布于水稻(Oryza stativa L.)12条染色体上的15对SSR(simplej sequence repeats)引物，对18种中科院成都生物所培育的四倍体水稻和9种大面积种植的二倍体水稻进行了SSR多态性分析．11对具多态性的引物共检测到33条多态性条带，平均每对引物检测到3个等位基因．研究结果发现，所用同源四倍体水稻的基因组与二倍体水稻基因组大部分相同，只是在某些位点上具有差异，并筛选出部分SSR标记来区分二倍体与其同源四倍体．本研究还对二倍体与同源四倍体之间遗传差异的原因进行了初步的探讨．图2表2参12
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| 关 键 词： | ssR 水稻 同源四倍体 |
| 修稿时间： | 2004年2月10日 |
GENETIC VARIATION OF TETRAPLOID AND DIPLOID RICE REVEALED BY MICROSATELLITE MARKS |
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| Abstract: | Fifteen SSR (simple sequence repeats) primers, which disperse on 12 chromosomes in rice, were used to detect genetic diversity among eighteen tetraploid and nine diploid rice varieties. Those primers detected 33 alleles, and the average number of alleles per SSR locus was 3, with a range from 2 to 5. The result preliminary showed that most genomes of the tetraploid and diploid rice were the same except some sites .SSR could be used to differentiate diploid from tetraploid. Besides, the genetic difference between tetraploid and diploid rice was also studied. Fig 2, Tab 2, Ref 12 |
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| Keywords: | SSR Rice Tetraploid |
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