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| 我国土壤中苏云金芽孢杆菌的分离与基因型的鉴定 | |
| 引用本文： | 罗兰,谢丙炎,袁忠林,杨之为.我国土壤中苏云金芽孢杆菌的分离与基因型的鉴定[J].应用与环境生物学报,2005,11(6):756-759. |
| 作者姓名： | 罗兰 谢丙炎 袁忠林 杨之为 |
| 作者单位： | 1. 西北农林科技大学植物保护学院,陕西,杨陵,712100;中国农业科学院蔬菜花卉研究所,北京,100081;莱阳农学院植物保护系,山东,莱阳,265200 2. 中国农业科学院蔬菜花卉研究所,北京,100081 3. 莱阳农学院植物保护系,山东,莱阳,265200 4. 西北农林科技大学植物保护学院,陕西,杨陵,712100 |
| 基金项目： | 国家重点基础研究发展规划项目(2002CB111400)，国家自然科学基金项目(30270236)~~ |
| 摘 要： | 从我国20个省市采集176份土壤中分离出苏云金芽孢杆菌51株.以cry1,cry2,cry3,cry4,cry-n,cry7,cry8,cry11,cry13和cyt的引物作PCR分析.结果表明:4株含有cry4,12株含有cry7,4株含有cry8,4株含有cry1,2株同时含有cry1和cry2,27株无PCR产物.未检测到其它基因型.经电镜扫描伴胞晶体形态多种多样,主要有菱形、球形、方形、多边形和不规则形.图3表3参15 |
| 关 键 词： | 苏云金芽孢杆菌 分离 聚合酶链式反应 伴孢晶体 |
| 收稿时间： | 2004-10-13 |
| 修稿时间： | 2005-01-04 |
IDENTIFICATION OF CRY- TYPE GENE AND ISOLATION OF BACILLUS THURINGIENSIS FROM SOIL IN CHINA |
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| LUO Lan,XIE Bingyan,YUAN Zhonglin,YANG Zhiwei.IDENTIFICATION OF CRY- TYPE GENE AND ISOLATION OF BACILLUS THURINGIENSIS FROM SOIL IN CHINA[J].Chinese Journal of Applied and Environmental Biology,2005,11(6):756-759. | |
| Authors: | LUO Lan XIE Bingyan YUAN Zhonglin YANG Zhiwei |
| Abstract: | 51 strains of Bacillus thuringiensis were isolated from soils in 20 provinces of China. The 51 Bt strains were identified by PCR method with general primers of cry1, cry2, cry3, cry4, cry-n, cry7, cry8, cry11, cry13 and cyt genes. The results showed that 4 strains of them contained cry4 type gene, 12 strains contained cry7, 4 strains contained cry8, 4 strains contained cry1, respectively, while 2 strains contained both cry1 and cry2, but 27 strains did not give any PCR product. Furthermore, none of them contained cry3, cry-n, cry11, cry13 and cyt type genes. Observed by scanning electron microscope, these Bt parasporal crystal shapes were bipyramidal, round, cuboidal, polygon and abnormity. Fig 3, Tab 3, Ref 15 |
| Keywords: | Bacillus thuringiensis isolation PCR analysis parasporal crystal |
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