伯克氏菌对水稻种子萌发及初生幼苗耐镉性的影响

在无菌条件下研究了伯克氏菌对镉胁迫下水稻种子萌发及初生幼苗耐镉性的影响。结果表明：伯克氏菌D54能在含500mg·L^-1Cd的培养基中正常生长,显示出极强的耐镉能力。在50 mg·L^-1的镉胁迫下,接种伯克氏菌能显著提高水稻种子的萌发率,但在其他镉处理下,接种伯克氏菌对种子萌发无显著影响。接种伯克氏菌对水稻种子的发芽指数没有影响,但在10 mg·L^-1Cd处理下,接种伯克氏菌能显著提高水稻种子的活力指数和初生幼苗的根长、根表面积、根尖总数和耐性系数。接种伯克氏菌对初生幼苗的芽鲜重无影响,但能显著提高根鲜重。在50 mg·L^-1和100 mg·L^-1的高浓度镉处理下,接种伯克氏菌对根系生长没有影响。

Effects of Burkholderia on Rice Seed Germination and Cd-tolerance of Rice Seedlings

We studied the effect of Burkholderia sp.D54 on rice seed germination and Cd-tolerance of rice seedlings under gnotobiotic Cd stress. The results showed that Burkholderia sp.D54 could grow normally under 500 mg·L^-1Cd stress. Inoculation of Burkholderia sp.D54 significantly increased rice seed germination rate under 50 mg·L^-1Cd stress,but it had no effect on germination index of any Cd treatment. Inoculation with Burkholderia sp.D54 could significantly improve vigour index of rice seeds and total root length,surface area,tip numbers and tolerance index of rice seedlings under 10 mg·L^-1Cd stress. Inoculation of Burkholderia sp.D54 could promote the growth of rice roots,but had no effect on the shoot. There were no significant difference of root length,surface area,tip numbers between inoculated and uninoculated one under 50 mg·L^-1and 100 mg·L^-1Cd stress.