Biomass from phytopathogens and culture conditions improve Penicillium chrysogenum antimicrobial activity and antifungal compounds production |
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Authors: | Sandra Pacios-Michelena Cristóbal N. Aguilar González Raúl Rodríguez Herrera Olga B. Alvarez-Perez Mónica L. Chávez González Roberto Arredondo Valdés Juan A. Ascacio Valdés Mayela Govea Salas Anna Iliná |
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Affiliation: | 1. Food Science Research Department, Faculty of Chemical Sciences, The Autonomous University of Coahuila, Saltillo, Coahuila, Mexico;2. GreenCorp Biorganiks de Mexico S.A de C.V., Saltillo, Coahuila, Mexico |
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Abstract: | The present study evaluated the effect of culture conditions and phytopathogenic strain co-culture on the production of antimicrobial metabolites and antifungal activity of Penicillium chrysogenum R1, which PCR identified. Antimicrobial activity was determined using the Hunter-Hunter experimental design with three factors (pH, incubation temperature, and inoculum, at two levels each). The antifungal metabolites, β 1-3 glucanase and chitinase, produced in the presence of live and inactivated Fusarium oxysporum Fsox C11 biomass, were evaluated using HPLC-MS and GC-MS. Results showed that P. chrysogenum inhibited the growth of five phytopathogenic fungal strains, and the most significant inhibition was observed for F. oxysporum Fsox C11. The best conditions to achieve the highest antifungal activity of the cell-free extract were pH 7, 28°C, 1 × 106 spores/mL, and 144 h of fermentation, observing 86% inhibition of F. oxysporum Fsox C11 growth. Production of antifungal metabolites such as 1,4-benzoquinone imine, viridicatic acid, phenol-5-methyl-2-(1-methyl ethyl), and hydrolytic enzymes β 1-3 glucanase and chitinase was detected. The results define the perspective in designing new processes and products for biocontrol phytopathogens. |
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Keywords: | antagonist antimicrobial biological control Fusarium oxysporum penicillium |
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