Evaluation of chlorophyll fluorescence, in vivo spectrophotometric pigment absorption and ion leakage as biomarkers of UV-B exposure in marine macroalgae |
| |
| Authors: | B Cordi M H Depledge D N Price L F Salter M E Donkin |
| |
| Institution: | (1) Plymouth Environmental Research Centre, University of Plymouth, Drake Circus, Plymouth PL4 8AA, Devon, England, GB;(2) Department of Biological Science, University of Plymouth, Drake Circus, Plymouth PL4 8AA, Devon, England, GB;(3) Centre for Science, Cornwall College, Pool, Redruth TRI5 3RD, Cornwall, England, GB |
| |
| Abstract: | The photosynthetic fluorescence ratio Fv:Fm, in vivo absorption spectra and ion leakage were evaluated as biomarkers of ambient and elevated UV-B (280 to 320 nm) exposure
of the intertidal alga Enteromorpha intestinalis (Chlorophyta) and the sublittoral alga Palmaria palmata (Rhodophyta). Measurements of thallus growth were also used to assess adverse biological effects. Ambient and elevated UV-B
significantly inhibited photosynthesis in both species. It was shown that the Fv:Fm ratio is a sensitive, non-specific general biomarker of UV-B exposure in both species. Moreover, the in vivo absorption of
what was tentatively identified as chlorophylls a and b as well as phycoerythrin and/or carotenoids, phycoerythrobilin and phycocyanin decreased in a dose-response dependent manner
and was associated with a decrease in growth rate in P. palmata. The intertidal alga E. intestinalis showed a greater degree of tolerance to UV-B exposure. These results indicate that changes in the Fv:Fm ratio together with reductions in in vivo pigment absorption could provide an early quantitative warning of the detrimental
effects of UV-B in marine macroalgae.
Received: 16 May 1997 / Accepted: 16 July 1997 |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
