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Experimental study on Cr(Ⅵ) reduction by Pseudomonas aeruginosa
引用本文:LIU Yun-guo,XU Wei-hu,ZENG Guang-ming,TANG Chun-fang,LI Cheng-feng. Experimental study on Cr(Ⅵ) reduction by Pseudomonas aeruginosa[J]. 环境科学学报(英文版), 2004, 16(5): 797-801
作者姓名:LIU Yun-guo  XU Wei-hu  ZENG Guang-ming  TANG Chun-fang  LI Cheng-feng
作者单位:DepartmentofEnvironmentalScienceandEngineering,HunanUniversity,Changsha410082,China
基金项目:TheNationalHi TechR&DProgram(863)ofChina(No .2 0 0 1AA6440 2 0 )
摘    要:Investigation on Cr( Ⅵ ) reduction was conducted using Pseudomonas aeruginosa. The study demonstrated that the Cr(Ⅵ) canbe effectively reduced to Cr( Ⅲ ) by Pseudomonas aeruginosa. The effects of the factors affecting Cr( Ⅵ ) reduction rate including carbon source type, pH, initial Cr(Ⅵ) concentration and amount of calls inoculum were thoroughly studied. Malate was found to yield maximum biotransformation, followed by succinate and glucose, with the reduction rate of 60.86%, 43. 76% and 28.86% respectively. The optimum pH for Cr( Ⅵ ) reduction was ?.0, with reduction efficiency of 61.71 % being achieved. With the increase of initial Cr(Ⅵ) concentration,the rate of Cr(Ⅵ) reduction decreased. The reduction was inhibited strongly when the initial Cr(Ⅵ) concentration increased to 157 mg/L. As the amount of cells inoculum increased, the rate of Cr( Ⅵ ) reduction also increased. The mechanism of Cr( Ⅵ ) reduction and final products were also analysed. The results suggested that the soluble enzymes appear to be responsible for Cr (Ⅵ) reduction by Pseudomonas aeruginosa, and the reduced Cr( Ⅲ ) was not precipitated in the form of Cr(OH)3.

关 键 词:Cr 铬 假单胞菌 铜绿菌素 环境保护 毒性
收稿时间:2003-10-30
修稿时间:2003-12-12

Experimental study on Cr (V) reduction by Pseudomonas aeruginosa
Liu Yun-Guo,Xu Wei-Hua,Zeng Guang-Ming,Tang Chun-Fang,Li Cheng-Feng. Experimental study on Cr (V) reduction by Pseudomonas aeruginosa[J]. Journal of environmental sciences (China), 2004, 16(5): 797-801
Authors:Liu Yun-Guo  Xu Wei-Hua  Zeng Guang-Ming  Tang Chun-Fang  Li Cheng-Feng
Affiliation:Department of Environmental Science and Engineering, Hunan University, Changsha 410082, China.
Abstract:Investigation on Cr(V) reduction was conducted using Pseudomonas aeruginosa. The study demonstrated that the Cr(VI) can be effectively reduced to Cr(III) by Pseudomonas aeruginosa. The effects of the factors affecting Cr(VI) reduction rate including carbon source type, pH, initial Cr(VI) concentration and amount of cells inoculum were thoroughly studied. Malate was found to yield maximum biotransformation, followed by succinate and glucose, with the reduction rate of 60.86%, 43.76% and 28.86% respectively. The optimum pH for Cr(VI) reduction was 7.0, with reduction efficiency of 61.71% being achieved. With the increase of initial Cr(VI) concentration, the rate of Cr(VI) reduction decreased. The reduction was inhibited strongly when the initial Cr(VI) concentration increased to 157 mg/L. As the amount of cells inoculum increased, the rate of Cr(VI) reduction also increased. The mechanism of Cr(VI) reduction and final products were also analysed. The results suggested that the soluble enzymes appear to be responsible for Cr(VI) reduction by Pseudomonas aeruginosa, and the reduced Cr(III) was not precipitated in the form of Cr(OH)3.
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