Whole genome amplification from a single cell: a new era for preimplantation genetic diagnosis |
| |
Authors: | Serdar Coskun Osama Alsmadi |
| |
Institution: | 1. Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia;2. Arabian Diagnostic Laboratories, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia |
| |
Abstract: | Preimplantation genetic diagnosis (PGD) is a technique used for determining the genetic status of a single cell biopsied from embryos or oocytes. Genetic analysis from a single cell is both rewarding and challenging, especially in PGD. The starting material is very limited and not replaceable, and the diagnosis has to be made in a very short time. Different whole genome amplification (WGA) techniques have been developed to specifically increase the DNA quantities originating from clinical samples with limited DNA contents. In this review, currently available WGA techniques are introduced and, among them, multiple displacement amplification (MDA) is discussed in detail. MDA generates abundant assay-ready DNA to perform broad panels of genetic assays through its ability to rapidly amplify genomes from single cells. The utilization of MDA for single-cell molecular analysis is expanding at a high rate, and MDA is expected to soon become an integral part of PGD. Copyright © 2007 John Wiley & Sons, Ltd. |
| |
Keywords: | whole genome amplification multiple displacement amplification primer extension preamplification degenerate oligonucleotide primed PCR preimplantation genetic diagnosis |
|
|