| 超声波树脂联用法提取活性污泥中的胞外聚合物 |
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| 引用本文： | 李绍峰,王宏杰,王雪芹,董文艺,崔崇威.超声波树脂联用法提取活性污泥中的胞外聚合物[J].安全与环境学报,2006,6(5):11-13. |
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| 作者姓名： | 李绍峰 王宏杰 王雪芹 董文艺 崔崇威 |
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| 作者单位： | 深圳职业技术学院建工系,深圳,518055;哈尔滨工业大学深圳研究生院,深圳,518055;哈尔滨工业大学市政环境工程学院,哈尔滨,150090 |
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| 基金项目： | 广东省自然科学基金
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教育部春晖计划项目 |
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| 摘 要： | 考察了超声波树脂联用法提取活性污泥中胞外聚合物的影响因素,确定了最佳提取条件.试验结果表明,树脂量为60 g/g VSS,在摇床振摇强度250 r/min下处理4 h,再进行超声处理2 min,EPS提取量接近理论含量,即污泥干重的15%,与阳离子树脂法相比,EPS提取物中DNA的百分含量较低,即对细胞的破坏程度更小,说明超声波树脂联用法稳定、高效,是一种较佳的提取方法.
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| 关 键 词： | 环境工程 超声波 树脂 活性污泥 胞外聚合物 |
| 文章编号： | 1009-6094（2006）05-0011-03 |
| 收稿时间： | 2006-03-10 |
| 修稿时间： | 2006年3月10日 |
New method on extracting ultrasonic joint cation resin EPS |
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| LI Shao-feng,WANG Hong-jie,WANG Xue-qin,DONG Wen-yi,CUI Chong-wei.New method on extracting ultrasonic joint cation resin EPS[J].Journal of Safety and Environment,2006,6(5):11-13. |
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| Authors: | LI Shao-feng WANG Hong-jie WANG Xue-qin DONG Wen-yi CUI Chong-wei |
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| Abstract: | This paper aims to introduce a new method for extracting the ultrasonic joint cation resin EPS by analyzing their influencing factors and the optimum extraction conditions.The experimental results of the authors indicate that when the specimen had been stirred for 4 h at frequency 250 r/min with the cation resin dose getting to 60 g/g VSS,the EPS extraction would be getting close to its theoretical content(15% of dry sludge)if treated by ultrasonic for 2 min.As compared with ordinary cation resin extraction method,the DNA percentage of EPS is lower,which indicate that the cell decomposed degree is much lower than the latter.All of this may suggest that the ultrasonic joint cation resin method of EPS extraction enjoys high efficiency and reliability and therefore is comparatively advantageous than the ordinary EPS ordinary extracting methods. |
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| Keywords: | environmental engineering ultrasonic cation resin activated sludge EPS |
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