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Proving the Authenticity of Ancient DNA by Comparative Genomic Hybridization |
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| Authors: | S. Hummel B. Herrmann J. Rameckers D. Müller K. Sperling H. Neitzel H. Tönnies |
| Affiliation: | Historische Anthropologie und Human?kologie, Institut für Zoologie und Anthropologie, Universit?t G?ttingen, Bürgerstrasse 50, D-37073 G?ttingen, Germany e-mail: shummel1@gwdg.de, Tel.: +49-551-393642, DE S?uglingsklinik, Klinikum Chemnitz, Flemmingstrasse 4, D-09009 Chemnitz, Germany, DE Institut für Humangenetik, Molekulare Zytogenetik, Charité, Campus Virchow Klinikum, Humboldt Universit?t, Haus H, Augustenburger Platz 1, D-13353 Berlin, Germany, DE |
| Abstract: | In PCR-supported amplification of ancient, degraded DNA, contamination with contemporary DNA can lead to false-positive results, which frequently give rise to discussions in which the mere existence of ancient DNA is doubted. Our confirmation of ancient DNA using comparative genome hybridization (CGH) eliminates these doubts. Unlike PCR methods, CGH requires no amplification of the DNA to be analyzed if adequate amounts of specimen DNA is used. Thus, false results traceable to contaminations are practically ruled out. The examples provided here prove the authenticity of ancient DNA for a 250-year-old and a 3000-year-old sample. At the same time, the CGH of ancient DNA offers the chance to gain insight into the pattern of DNA degradation and to monitor the preservation of certain chromosomal segments. Received: 10 May 1999 / Accepted in revised form: 29 July 1999 |
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