A Simple Assay for Monitoring Cellulose in Paper-Spiked Soil |
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Authors: | Terrence R Green and Radu Popa |
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Institution: | (1) TGA LLC, 13851 Verte Court, Lake Oswego, OR 97034, USA;(2) Biology Department, Portland State University, PO Box 751, Portland, OR 97207, USA |
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Abstract: | We describe a simple method for measuring cellulose in soil. We used this method to measure the recovery of shredded office
paper and pure medium fiber cellulose added to a Helvetia silt loam clay soil. This method consists of solvating cellulose
from soil with 77% H2SO4 and analysis of the cellulose recovered by the phenol-sulfuric acid carbohydrate assay. Unlike previous related methods the
modifications we propose allow good recovery of cellulose (~99%) and eliminate the need to autoclave the samples. We verified
this method on soil spiked with up to 12% cellulose and found a good linear relationship between the amounts of cellulose
added relative to that recovered. With proper fragmentation and dilution of the acid-treated soil samples, higher concentrations
can be easily measured. We propose this technique as a robust and high throughput means to monitor the degradation of cellulose
in paper spiked soil. |
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